Journal of Stem Cell Research & Therapy

Aarya Hari

Publications

• Research Article
  Production of Good Manufacturing Practice Grade Equine Adiposederived Mesenchymal Stem Cells for Therapeutic Use
  Author(s): Shashank Gowda, Aarya Hari, Basavaraj Chougule, Manoj Kumar Reddy, Abhishek Chandanan, Minita Sodhi, Nicole Koshy, Lyle Fonseca and Satish ToteyShashank Gowda, Aarya Hari, Basavaraj Chougule, Manoj Kumar Reddy, Abhishek Chandanan, Minita Sodhi, Nicole Koshy, Lyle Fonseca and Satish Totey
  
  Mesenchymal stem cells (MSC) are currently being evaluated in equine clinical studies for their potential to treat various disorders and injuries. Studies have shown that both autologous and allogeneic stem cells appear to be safe. However, an efficient cell expansion method that is reliable and cost-effective is warranted to provide off-the-shelf clinical grade stem cells. Our study aimed to determine optimum culture conditions for efficient large-scale stem cell expansion. We produced cGMP standard equine adipose derived mesenchymal stem cells on a large scale. Five different medium combinations—Dulbecco’s modified Eagle’s medium-knockout (DMEM-KO), alpha modified minimum essential medium (α-MEM), 50:50 DMEM-KO/α-MEM, 75:25 DMEM-KO/α-MEM and 25:75 DMEM-KO/α- MEM—at seeding densities of 1000, 2000, 3000, 4000 and 5000 cells/cm2, were us.. View More»
  DOI: 10.4172/2157-7633.1000154

  Abstract PDF