Saeid Navabpour, Dariush Ebadi Almas, Haniyeh Kafi and Saeed Mahdavi Aghdam
During the past few decades, the stratospheric ozone reduction problem has stimulated remarkable research on higher plant responses to UV-B radiation. However, little of this work has addressed the reproductive biology of plants. The purpose of this study was to investigate the effects of UV-B radiation on reactive oxygen species (ROS) accumulation and antioxidant defense system in relation to germination and tube growth of canola (Brassica napus L) pollen. Our results illustrate that increased UV-B radiation decreased the pollen germination rate and tube length in vitro. Production of superoxide anion radical (O2) and hydrogen peroxide (H2O2) increased by UV-B radiation treatment, and their accumulation resulted in lipid peroxidation. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were decreased by enhanced UV-B radiation. The increased ROS and lipid peroxidation, as well as decreased antioxidant activities may be attributed to the effects of UV-B radiation on pollen germination and tube growth.
Published Date: 2021-03-31; Received Date: 2021-02-02