Anatomy & Physiology: Current Research
Open Access

Abstract

The Role of Anticoagulant Type in Modulating the Effect of Laser Irradiation on Blood Rheology

Eman S

Background: Evaluating blood optical and rheological considerations plays a role vital to various routine clinical investigations and therapeutic processes, and such evaluation is a superlative model for identifying the interaction mechanisms between biological tissues and low levels of laser irradiation. The purpose of this research was to investigate the in-vitro influence of laser radiation on certain blood indices.

Material and Methods: Blood samples were obtained from 40 healthy volunteers, and 30 samples were divided into 4 groups, 1 of which was the control and the other 3 of which were exposed to 3 doses of green laser while using ethylene diamine tetra acetic acid (EDTA) as an anticoagulant. The remaining 10 samples were anticoagulated using heparin and grouped into 2 aliquots, 1 of which was the control and the other of which was exposed to a single dose of green laser radiation. The test achieved a wavelength of 532 nm and a beam-spot diameter of 4 mm. The irradiation times used were 1.8, 3.7, and 6.2 sec, resulting in irradiation doses of 1.5, 3 and 5 J/cm2 respectively.

Results: Green laser irradiation promoted significant alterations in the mean corpuscular volume (MCV) of erythrocytes regardless of the type of anticoagulant. It also increased the white blood cell (WBC) count when EDTA was used as the anticoagulant, but not when heparin was used.

Conclusion: The type of anticoagulant used may alter the effect of lasers on certain blood parameters.