Biochemistry & Pharmacology: Open Access
Open Access

Abstract

Technical Aspects of 3-Dimentional Culture of Mammalian Cells

Ahmed Mohamed Al-Abd and Fahad Ahmed Al-Abbasi

Three dimensional-tissue culture systems of human solid tumor cells has been implemented for effective
chemotherapeutic screening and to avoid the disadvantages of the over simplified monolayer tissue culture system. The
influence of tumor microenvironment to tumor therapeutics and screening of new anticancer drugs has been found to
significant. The complex tumor microenvironment is poorly represented in the simple monolayer tissue culture system.
The 3D culture models closely imitate several in vivo conditions of solid tumors in terms of geometry and expression of
microenvironment. . However, several technical obstacles might discourage the use of this culture model. In the current
mini-review, we are presenting the exact protocol for culturing mammalian cell as three dimensional tissue culture
systems (multicellular spheroids and multicellular layers) and how to utilize these models for anti-cancer assessment.