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Yili Ding, Chamakura VNS Varaprasad, Saeed El- Ashram, Jiedan Liao, Nan Zhang and Bingyun Wang
Carbohydrate recognition has been shown to be involved in various biological processes. E-selectin is expressed on the endothelial cells stimulated by cytokines, such as IL-1β or TNF-α at inflammatory sites and plays an important role in the transport of neutrophils to inflammatory sites. The tetrasaccharide SLex is distributed on the surface of neutrophils and has been shown to be a ligand recognized by E-selectin. SLex and its derivatives can block the interaction between SLex and E-selectin and will be useful as the anti-inflammatory and anti-tumor agents.
Random fucosylation on a partially protected sialyl LacNAc trisaccharide, resulted in the corresponding glycosylated products as a mixture. After careful purification, debenzylation, deacetylation, alkaline hydrolysis and further purification on C-18 column, a tetrasaccharide library containing five sialyl lewis X related tetrsaccharides was obtained, and the results were confirmed by proton NMR study.
Random fucosylation was demonstrated as efficient method to generate sialyl related tetrasaccharide library.