A benzylamine supported palladium (II) complex has been synthesized and characterized using different spectroscopic techniques, such as FTIR, UV/Vis., 1H, 13C NMR and LCMS. The semi crystalline nature of the complex was investigated through powder XRD and SAED pattern analysed by HRTEM. The in vitro biological study against breast cancer cell lines MCF-7 and MDA-MB-231 was not much effective but their strong DNA binding activity (DBA) compared to free ligand suggested them as a good DNA binder and makes them interesting against other cancerous cell lines. The DBA further supported by physicochemical studies such as surface tension and viscosity of complex+DNA which inferred the disruption and intercalation of DNA and complex. The scavenging activity (SA) also proves the anticancer nature of complex, in this context, the scavenging activity of complex was determined through DPPH• free radical and the result indicates good antioxidant behaviour of complexes.