Three new Macrocyclic Hydrazone Schiff bases were synthesized by condensation of intermediate compounds: 1,6- bis (2-formylphenyl) hexane and glutaraldehyde with both dihydrazide of isophthalic acid and dihydrazide of terephthalic acid. Identification of these macrocyclic Schiff bases ligands (V, VI, VII). The Schiff bases were checked by different spectral technique (LC-MS, 1H-NMR, IR, elemental analyses). The new Macrocyclic Hydrazone Schiff Bases were studied for antibacterial activities against Gram positive (Bacillus subtilis and Staphylococcus aureus) and Gram negative (Salmonella typhi and Escherichia coli). The compound ligands exhibited a variable activity of inhibition on the growth of the bacteria.