In this research, extracts of Elaeagnus angustifolia leaf is used to reduce the lifetime of silver nanoparticles for the first time. The photosynthesized nanoparticles are homogeneously dispersed into Alkyd resin with 0.5 and 1.0 weight percentage. The two-stage Alcoholysis-poly esterification method was used to synthesis Alkyd resin in 210°C. First, mono glyceride is formed from triglyceride and glycerol, and then Alkyd resin is produced from mono glyceride and phthalic anhydride. The prepared samples including pure Alkyd resin and Alkyd resin with 0.5 and 1.0 weight percentage of silver are investigated by FT-IR, UV-vis, SEM, TGA and XRD analysis. In addition, to study on the antibacterial effects of the loaded nanoparticles, growth and reproduction of two well-known bacteria, i.e., Staphylococus aureus and Klebsiella pneumoniae, in a nutrient agar containing resultant disk-like nanocomposites are evaluated. The results showed that nano composites containing antibacterial silver has non-growth bacteria halo. While pure Alkyd resin don’t have this capability. The more silver amount in polymer matrix, the less bacteria resistance against it and the non-growth halo diameter increases. Finally, the presence of silver nanoparticles in polymer matrix will lead to anti-bacterial property which can be used in the containers keeping food stuff, medical and dental equipment.