Current Synthetic and Systems Biology
Open Access

Abstract

?MYB-Related Protein P-Like? is the Candidate Gene Homologus to ZmMYBIF35 R2R3-Transcription Factor in Wheat (Triticum aestivum L.) for Anythocyanin Pathway Modifications

Tayyaba Noor*, Faisal Saeed Awan

Transcription factors belonging to R2R3 family play an essential role in the regulation of secondary metabolites and during stress conditions. Diverse number of such transcription factors has been identified but very few have been characterized in monocots. ZmMYB_IF35 have been characterized previously in Zea mays in regulation of anthocyanin biosynthetic pathway. This study aims at characterizing the R2R3-MYB transcription factors similar to ZmMYB-IF35 in wheat for the purpose of regulation of flavonoid/anthocyanin pathway through computational tools. ‘MYB-related protein P-like’ (C₁₆₁₈H₂₅₆₅N479O₅₃₄S₁₅) is a negatively charged protein, composed of 346 amino acids with molecular weight of 37752.95 (da) and isoelectric point of 5.19. It is highly thermostable and is localized in the nucleus. Gene responsible for its transcription is located on chromosome 4B on LOC119296349 in wheat (Triticum dicoccoides). Significant portion of the protein was found to be composed of random coils (50%) and small nonpolar residues. From transmembrane topology predictions we showed that, N-terminal of the protein constitutes of all transmembrane helixes. From gene ontology predictions, biological function was showed to be regulation of RNA biosynthesis process majorly and it binds to the nucleic acids/DNA. No phosphorylation was shown as post translational modification apart from glycosylation between 150-200 amino acid residues. Interestingly, PEST sequences were also found within the glycosylation rich region. Ile-58, Gly-64, Leu-77, Glu-103, Glu-34 were shown to be functional residues that can be mutated at the catalytic pocket. The likely centroid ligands that can bind to the binding site 180-184 (residues) and 225-228 (residues) were shown to be dopamine (DA382; DA893) and ruthenium (RA914) with intermediating role in binding to DNA or chromatin. A total of three superfamilies were predicted; PLN03212, REB1 superfamily and SANT superfamily were predicted with SANT MYB-DNA binding superfamily with highest significance near N-terminal of sequence. Our results provide novel insights on the characterization of ‘MUB-related protein P-like’ in Triticum dicoccoides homologous to Zea mays ZmMYBIF35 for the regulation of flavonoid/anthocyanin biosynthesis pathway in wheat.

Published Date: 2025-04-09; Received Date: 2023-11-21