Journal of Nanomedicine & Biotherapeutic Discovery
Open Access

Abstract

Mesoporous Nano-carbon Particle Loaded Fisetin has a Positive Therapeutic Effect in a Murine Preclinical Model of Ovalbumin Induced Acute Allergic Asthma

Shinjini Mitra, Pramathadhip Paul, Kaustab Mukherjee, Silpak Biswas, Mayank Jain, Aryabaran Sinha, Nikhil Ranjan Jana and Ena Ray Banerjee

Inflammation is a complex process initiated by several factors ranging from bacterial infection and chemical injury to environmental pollution that causes cell injury or death, release of inflammatory mediators which further trigger the up-regulation of other pro-inflammatory cytokines and chemokines as well as sequestration of specific immunoglobulin molecules. Th2 inflammation is an allergenic reaction by primed T and B cells and during manifestation of acute asthma, this immune reaction takes the form of super-activated macrophages, eosinophils, neutrophils and an array of Th2 skewed inflammasome development. An anti-inflammatory molecule should be able to potently reduce or inhibit one or more of the aforementioned traits. Several organic and inorganic substances have been studied to evaluate their potential as anti-inflammatory molecules, among which nanotechnology has emerged as a promising aid to anti-inflammatory activities. Nanoparticle-incorporated mesoporous carbon particles have a wide range of applications in controlled drug delivery. These nano particles are capable of delivering drugs inside the cell, even in a sub-cellular organelle specific manner. In this study, inflammation is initiated by using different classes of pro-inflammatory stimuli, such as OVA (chicken ovalbumin), LPS (lipopolysaccharide) and TG (thioglycollate), and by applying such nano-concoctions, the anti-inflammatory activities of certain compounds are assessed in in vivo models. The anti- inflammatory action of a novel drug made from extract of strawberry (fisetin) has been evaluated in an in vivo model of acute allergic asthma in Balb/c mice. Also, attempts are being made to narrow the delivery window within the cell, by loading the drug onto the constructed mesoporous carbon nanoparticle. It has been found to maintain cell viability significantly by reducing oxidative and nitrosative stress and ameliorating the overall development of composite asthma phenotype in this murine preclinical model.