Seventy samples were collected from male individuals suffering from infertility either primary or secondary, as well as 35 samples were collected from fertile persons served as a control group.
Criteria referred to by WHO for semen parameter were applied through-out the course of the study, these criteria includes: Age, volume of the specimen, total sperm count/mL, morphology of sperm, pH of the specimen, liquefaction time, and activity of sperm. The effect of oxygen reactive species was estimated by measuring of the concentration of malondialdihyde.
Computerized system (General Sperm Analyzer, motic type) was used to determine semen parameter mentioned above. Seminal plasma of liquefied samples were collected from all samples by centrifugation at 3000 rpm for 7 min, plasma were stored frozen (-80°C) utile use. Enzyme Linked Immune-Sorbent Assay technique (Elisa) was used to detect MDA level in seminal plasma.
Published Date: 2018-12-10; Received Date: 2018-11-27