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Abstract Background and objective: Sexual health is an important, however a neglected factor in patients with diabetes. Effects of diabetes mellitus (e.g. erectile dysfunction) and implication on sexual frequency and function among men with diabetes have not been well investigated. The current study aims to evaluate the sexual frequency, sexual function and their association with type 2 diabetes compared to that in an age-matched control group. Methods: In all, 50 married men with type 2 diabetes mellitus attending the outpatient endocrine clinic of Al- Zahraa University Hospital between March 2011 and March 2012 were selected. Glycosylated hemoglobin (HbA1C) and fasting plasma glucose (FPG) were measured. Sexual frequency and function were assessed by questionnaire. The scores in each item of sexual frequency and function were compared with those of 50 healthy non-diabetic controls. Results: Sexual frequency and function scores for sexual drive, orgasm, overall satisfaction and arousal domains were decreased in the diabetic men (p<0.05). Age of the patients and duration of diabetes were negatively correlated with all items of sexual frequency and function. Body mass index (BMI), fasting plasma glucose, Glycated hemoglobin, education and employment status did not have a significant correlation with sexual frequency and function items. Conclusions: Diabetes significantly affects the sexual frequency and function of diabetic men. Age and duration of diabetes were the most powerful determining factors of sexual frequency and function.