Hepatocellular carcinoma (HCC) is one of the major causes of cancer related death globally. Successful treatment of liver cancer remains a formidable challenge in modern drug development due to sub-therapeutic permeation of conventional chemotherapeutics to the proper site of action along with its toxic side effects. Ligand conjugated nanoliposomes are an emerging formulation in the treatment of cancer. Flavonoids are abundantly present in fruits and vegetable are believed to carry preventive and therapeutic potential against cancer. Here we have optimized the aptamer conjugated nanoliposomes which is encapsulated with a bio-flavonoid and we studied its preferential uptake and efficacy on liver cancer. Various physiochemical and biopharmaceutical characterization studies such as drug-excipients interaction, surface morphology, energy dispersive X-ray analysis, zeta potential, in vitro drug release and cytotoxicity along with cellular uptake were conducted. Drug loaded nanoliposomes (D-NL) and aptamer conjugated drug loaded nanoliposomes (D-NL-A) showed 3.51±0.26% and 3.23±0.05% drug loading values, respectively. Average diameters (z-average) of the nanoliposomes were within 100 nm; it was also showed negative zeta potentials along with smooth surface and intact lamellarity. Predominant uptake of both the types of nanoliposomes was visualized. In vivo pharmacokinetic and biodristribution study in swiss albino rats showed that the drug availability significantly increased in carcinogenic liver upon (D-NL-A) treatment in comparison with free drug and (D-NL). Ligand conjugated nanoliposomal drug delivery substantially controlled the severity of hepatocellular carcinoma and could be a future hope for lingering the survival in hepatic cancer patients.
Published Date: 2020-11-30; Received Date: 2020-11-20