Journal of Proteomics & Bioinformatics
Open Access

Abstract

Computational Analysis of Mutations in Colon Cancer Genes Reveals a Possible Role of Micro Satellite in Mutagenesis

Allam Appa Rao, G R Sridhar, Suresh B Mudunuri, E Vamsidhar, Gunna Kishore

Computer science is a subject, which deals with the manipulation of data so that new data, implicit in the original, appear in a useful form. We have used the analogy of genome analysis and VIRUS (vital information recourse under siege) and analyzed MLH1, MSH2 and MSH6 gene which play an important role in repairing mistakes made in DNA replication in colon cancer. If the MLH1, MSH2, MSH6 proteins are mutated and therefore don’t work properly, the replication mistakes are not repaired, leading to damaged DNA. The information of all the experimentally proven mutations were collected and analyzed using bioinformatics tools and software programs. We tried to find out whether the presence of or simple sequence repeats in the MLH1, MSH2, MSH6 gene has any significance in the generation of these mutations and checked whether these mutations are fallen in the regions of those microsatellites and if so is there any significance of these microsatellites in the functional domains of the each gene. Our analysis reveled that 3 of the 10 mutations of the MLH1 gene and all the 10 mutations of the MSH2 gene and the one mutation in the MSH6 gene that are existing in the microsatellite regions are fall.en in the domain regions of the respective genes and thus indicating a positive role of microsatellites in mutagenesis.