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Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Abstract

Characterisation and Structural Dynamics of Differentially Expressed Proteins of Probiotic Eschericia coli Nissle 1917 in Response to Cocos nucifera Sap

Chandrasekhar K, Sreedevi B, Sreevani S, Dileep A, Lebonah D, Seshapani P and Pramoda kumari J

The aim of the present study was to evaluate and analyse the expressed protein structures in response to cocoti sap treated probiotic E. coli Nissle 1917 by MALDI-TOF-MS. The expressed protein spots were separated by 2-D gel electrophoresis and spots were digested with trypsin enzyme for MALDI analysis. Based on their massto- charge ratio, the expressed proteins sequences were collected from Mascot search data. The sequence are analysed by phyre-2 software for homology modelling. The predicted structures were also validated by Rampage software. Of the expressed proteins, only five proteins showed good structural validation in sap treated probiotics. It was concluded that the expressed protein profiles were analysed by 2D-PAGE and MALDI to understand the stress mechanisms of cocoti sap in probiotic E. coli Nissle 1917. Stress mechanism induces many proteins they are involved in metabolic process, regulate gene expression, SOS regulation, transferase activity, purine metabolism and other related activities.

 

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