Plant products that are rich in antioxidant properties include grape, one of the world’s largest fruit crops and most commonly consumed fruits in the world. Grape extract comprises several bio-active compounds, mostly represented by polyphenols and phenolic acids. It could be an excellent alternative to toxic chemo drugs, with minimal side effects and economical. Grape extract exerts high antioxidant and anticancer activities against cell lines. It could be used to treat a number of cancers, including lung, colon, cervical, bladder and prostate cancers. In this paper, the antioxidant and anticancer activity of pulsed electric field (PEF) treated grape extract is investigated. HeLa cell line (cervical cancer) was used for this purpose. The results indicate more cell death due to PEF-treated extract compared to untreated one, indicating the potency of PEF-treated extract. This shows the potential of using PEF-treated grape extract as a possible anti-cancer drug.