Chemotherapy: Open Access
Open Access

Abstract

Alpha Fetoprotein Plays Antagonistic Role in Benzyl-Isothiocyanate Arresting Cell Cycle in Liver Cancer Cells

Mingyue Zhu, Wei Li, Xu Dong, Peng Zhou, Junli Guo and Mengsen Li

Background: To investigate the effect of alpha fetoprotein (AFP) on benzyl-isothiocyanate (BITC) arresting cell cycle in human liver cancer cells line in vitro, and explore the possible role mechanism of BITC inhibited proliferation of hepatocellular carcinoma (HCC) cells. Methods: In this study, we selected HCC cells lines, Bel 7402 and HLE for test. Fluorescent microscopy and Western blotting were applied to observe the transfected effect and expression of proteins; MTT assay the proliferation of HCC cells; Flow cytometry method was used to detect the cellular cycle; RNA interference was used to test and expressed vector constructed technology were performed to silence and induce expression of AFP, respectively. Results: Analysis showed BITC had a significant inhibitory effect on the proliferation of HCC cells in dosedependent manner, inhibitory effect was enhanced while Bel 7402 cells were transfected with AFP-siRNA vectors but attenuated in HLE cells while transfected with pcDNA3.1-afp vectors. The growth ratio of AFP-siRNA transfected Bel 7402 cells and pcDNA3.1-afp transfected HLE cells followed treated with 80 ?mol/L BITC were (42.43 � 4.92)% (P