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“All and None” Refining Strategy; Fishing Your Correct Protein from Proteomics Ocean | Abstract
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Abstract

“All and None” Refining Strategy; Fishing Your Correct Protein from Proteomics Ocean

Sameh Magdeldin, Yutaka Yoshida, Ying Zhang, Bo Xu, Eishin Yaoita and Tadashi Yamamoto

We have developed an easy-to use methodology for refining large extensible markup language (XML) - based proteomics dataset with a high stringent and simple approach using VBA- coded plug-in. A methodology we term it (All and None). Selections of targeted candidates differentially significant between compared groups were selected based on its appearance or absence followed by peptide screening with a novel and simple approach. By testing the reliability and efficiency of this method, All and None was confirmed to be an applicable process for initial screening of biological biomarkers in complex specimens and tissue extract.

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