Single Cell Imaging is a microscopy technique that will collect data from separate individual cells, as opposed to values averaged over population of cells. It reveals populations of genetically identical calls which often present heterogeneous phenotypes and it often relies on fluorescent molecules. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Recent progress in this field has led to the development of a number of genetically encoded fluorescent biosensors, which upon recombinant expression are able selectively to detect real-time changes in IP(3) in single live cells.Live-cell imaging and computational modelling are compatible techniques which allow quantitative analysis of cell signalling pathway dynamics. Non-invasive imaging techniques, based on the use of various luciferases and fluorescent proteins, trace cellular events such as gene expression, protein-protein interactions and protein localization in cells. By employing a number of markers in a single assay, multiple parameters can be measured simultaneously in the same cell.
Related Journals of Single Cell Imaging
Journal of Cell Signaling, Journal of Cellular & Molecular Pathology, Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology, Insights in Cell Science, Insights in Stem Cells, Cellular Signalling, Protein & cell, Cell, Molecular Cell, Developmental Cell, Cell Stem Cell, Cell Cycle