Pharmaceutica Analytica Acta

Therapeutic efficacy of Propol T in postsynthetic modifications of the ECM components in the course of thermally damaged tissues repair.

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Krystyna Olczyk, Paweł Olczyk, Katarzyna Komosinska-Vassev, Katarzyna Winsz-Szczotka and Lukasz Mencner

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Background: In the course of wound healing process metalloproteinases(MMPs)take part already in the inflammatory phasethroughout phagocytosis and process of killing bacteria or removing debris. MMPs participate also in the remodeling phasethereby modulating epithelialization, determining separation ofkeratinocytesfromthe basalmembranesand,supportingthe migration ofkeratinocytesthrough a network ofcollagen.During the next stage of remodeling process, angiogenesis,MMPs degrade the basal membranes, promote migration of the endothelial cells and releasegrowth factorssequesteredinECM.The crucial role of matrix metalloproteinasesduring wound healing phenomenon as well as the well - known sequence of MMPs changes during the different phases of wound repair, allowed to apply the experimental model of cutaneous wound repair in orderto evaluate the therapeutic efficacy of new natural products. The aim of the present study was to assess the Propol T therapeutic valuein comparison with silver sulfadiazine in the treatment of minor skin burns, inflicted on white domestic pigs. Material and methods: Contact burn wounds were inflicted according to the methods of Hoekstra et al. and Brans et al. The wounds were treated with apitherapeutic agent (propolis), silver sulfadiazine (SSD), propolis vehicle and physiological salt solution,twice a day, for 21 days.The assessment of MMPs content in the tissue material derived from healing post-burn wounds was made byimmunoenzymatic method. Results: An increased expression of MMP1 throughout ten days of experimental healing process, especially pronounced after Propol T application, was found. Mentioned changes preceded the reduction and furthernormalisation of MMP1 expression. Burn wounds treatment with SSD caused enhanced accumulation of MMP-1 in the matrix of thermal damage until the tenthday of the experiment, followed by the subsequent content reduction.It was also found that the accumulation of MMP3 in thermal damages increased after Propol T and SSD application. The most markedtissue expression of this enzyme was observed in the case of wounds managed with apitherapeutic agent. The fact which is of particular interest isthat the content of MMP-3 was reducedin the final phase(days15, 21) of burn healing process,after application of both apitherapeutic agent and silver sulfadiazine, although the mentioned reduction wasmore intensein thecase of natural product implementation.Increased dynamics of changes and markedly assigned increased content of both MMP1 and MMP-3 after Propol T applicationmay indicate the greater therapeutic efficacy of the natural agent in comparison with SSD, on the modulation of tissue MMPs? expression. Conclusion: The presented results confirm the therapeutic efficacy of propolis which is connected with generating of favorable biochemical environment supporting healing process.