Journal of Liver

The potential use of saliva and dried blood spot for viral hepatitis diagnosis

2^nd International Conference on Hepatology

May 09-11, 2016 Chicago, USA

Livia Melo Villar

Oswaldo Cruz Foundation, Brazil

Posters & Accepted Abstracts: J Liver

Abstract:

Hepatitis B and C viruses are responsible for the most of chronic liver disease worldwide. In order to conduct HBV and HCV diagnosis, it is necessary blood sample collection by venipuncture in order to obtain sera or plasma samples. However, venipuncture is difficult in some individuals like drug users, haemodialysis, obese and elder individuals. In addition, the transport of these samples from remote areas to laboratories could be difficult. This situation has led to development of methods for HBV and HCV diagnosis in alternative fluids, like saliva or dried blood spot (DBS) samples. We optimized a commercial immunoassay (EIA) for HBsAg detection among saliva samples demonstrating sensitivities and specificities higher than 90%. Using DBS samples along to EIA, sensitivity was 90.5%, 97.6%, and 78% for anti-HBc, HBsAg, anti-HBs assays, respectively. Specificity was 92.6%, 96.7%, and 97.3% for anti-HBc, HBsAg, and anti-HBs assays, respectively. HBV markers could be detected in DBS samples using EIA until 60 days of storage in room temperature. Anti-HCV assays using saliva and DBS samples showed sensitivity and specificity higher than 90% and DBS samples can be stored for a period of 117 days at room temperature. HBV DNA was also detected in artificially contaminated saliva while HCV RNA was detected, quantified and genotyped in saliva and DBS samples showing good concordance to paired sera samples. These results showed the usefulness of saliva and DBS to increase the access of diagnosis in remote areas or individuals with poor venous access.

Biography :

Email: liviafiocruz@gmail.com