Journal of Aquaculture Research & Development

The potential of Eucheuma serra lectin as immunomodulator in Vannamei shrimp by using the total hemocyte count (THC)

3^rd International Conference on Aquaculture & Fisheries

September 29-October 01, 2016 London, UK

Merdeka Agus Saputra, Shobrina Silmi Q T, Fariz Kukuh Harwinda, Sri Umida Setyaningsih, Kartiko Arif Purnomo and Woro Hastuti Satyantini

University of Airlangga, Indonesia

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

The declining of shrimp production from 209.648 tonnes in 2008 to 170.969 tonnes in 2009 in Indonesia was caused by several pathogens such as virus, bacteria and parasite infecting the shrimp. Eucheuma serra has several minerals, proteins and glucose or glycoprotein, where 80% of its protein consists of lectin. Lectin from E. serra (ESA) can recognize and bind with the sugar portion of other molecules from foreign particles with broad specificity and also functionate as immunomodulator activity that can stimulates the hemocyte production and activation. This research aims to identify the effect of lectin from E. serra to enhance the non-specific immune of the shrimp by using THC and to identify the optimal dosage. The research method used was complete randomized design with 5 treatments (2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm) and one control with 4 repetitions. The lectin administered with the injection in fourth segment and the depriving of hemocyte in day 0 was applied in order to identify the amount of hemocyte which can be produced after acclimation, whereas the depriving in day 6 was estimated, that the direct injection in bloodstream of shrimp can enhance hemocyte production and activation more effectively. The result showed that the highest hemocyte productivity was occurred in p4 treatment with 8 ppm of injected lectin dosage, accounting for 18.45 million cells/ml in average. Subsequently, p1 (2 ppm) and p4 (12 ppm) had a nearly similar amount of hemocyte, constituting to 14.82 million cells/ml and 15.03 cells/ml in average. In conclusion, the high immune response showed in 8 ppm of treatment with the rise of hemocyte approximately in an average of about 18.45 million cells/ml. It means that lectin from E. serra positively can be used as immunomodulator in vannamei shrimp.

Biography :

Email: merdeka.agussaputra@gmail.com