Journal of Clinical & Experimental Pharmacology

The development of pharmacological strategy of cell therapy and perspectives for the creation of highly selective drugs for regenerative medicine on the basis of alkaloids

World Congress on Pharmacology

July 20-22, 2015 Brisbane, Australia

Zyuz?kov G N1,2, Suslov N1, Zhdanov V V1, Udut E V1, Miroshnichenko L A1, Mina-kova M Y1, Udut V V1, 2 and Dygay A M1

Posters-Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Background: ?Pharmacological strategy of regenerative medicine? based on the princi-ple of imitating the activity of the natural regulatory systems is considered to be the most physiologic and promising approach to solve the problems of regenerative medi-cine. Purpose: It is necessary to assess the perspective of developing drugs for regenerative medicine on the basis of alkaloids. Materials & Methods: The diterpene alkaloids: napellin, zongorin, gipakonitin, meza-konitin, Z77 and others were studied on experimental models of skin wound, cytostatic myelosuppression, posthypoxic encephalopathy, cerebrovascular accident. Histological, functional, cultural, hematologic and other methods were used. Results: The alkaloids with different types of regenerative activity were revealed. Napellin, zongorin, gipakonitin showed the most expressed wound healing properties, zongorin and napellin ? erythro- and granulocytopoeisis-stimulating ones respectively, and Z77 ?cerebro protective one. It was found that a direct effect of these substances on the growth factor receptors of progenitor cells and an increase in the functional activity of tissue microenvironment elements were the mechanisms of their action. In this case, ?reserve? intracellular IKK, PKC1, PKB, PKA dependent directions NF- ?B- and ?38 ????- signaling, which don?t participate in the realization of SC growth potential in the optimal conditions of life, were involved in the transfer of stimulating signal to progenitor cells. Conclusion: Obtained results indicate a high perspective of developing selective drugs for regenerative medicine on the basis of detected alkaloids.