Journal of Clinical & Experimental Pharmacology

Testicular toxicity characters and mechanisms of hydroxyurea on rats

8^th World Congress on Pharmacology and Toxicology

July 24-25, 2017 Melbourne, Australia

Han Yan, Li Zhou, Yong-wei Luo and Zu-yue Sun

Shanghai Institute of Planned Parenthood Research, China

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Objective: Apoptosis plays a dominant role in both spontaneous spermatogenesis and germ cell death. This study was aimed to investigate the functions of related genes in testicular germ cell death induced by Hydroxyurea (HU). Method: Wild-type (WT) and FasL Transgenic (TG) DBA/C57BL mice were intraperitoneal injected with 400 mg/kg HU. 12 hours later, testes were collected. Histomorphology of testis was observed by stained with Periodic Acid Schiff (PAS). Apoptosis was assessed by TUNEL assay. mRNA and protein levels of related genes were evaluated by quantitative RT-PCR and Western blot respectively. Results: The 2�?2 factorial design comparative experiments between WT and TG mice showed that the TG mice exhibited a higher basal apoptotic index. The basal mRNA levels of Fas and FasL and protein levels of Fas, FasL, Caspase-3, Caspase-8 and Caspase-9 in TG mice were also higher than that in WT mice. 12 hours after injection of HU, the testicular tubules exhibited no significantly morphological changes but remarkably increased apoptosis index in both WT and TG mice, with the latter having the higher amplitude. Although HU up-regulated the mRNA of apoptosis related genes such as Fas and FasL in both TG and WT mice, the increased amplitude were more obvious in TG mice. By Western blot analysis, apoptosis related proteins Fas, FasL Caspase-3, Caspase- 8 and Caspase-9 were significantly increased in both WT and TG mice, with TG mice exhibiting a greater up-regulation. Conclusion: Germ cell apoptosis induced by HU treatment may be related to the FasL mediated signal transduction pathway.

Biography :

Han Yan has completed the masters in Quality Assurance from National Evaluation Centre for the Toxicology of Fertility Regulating Drug. She has published more than 20 papers in China and abroad reputed journals.

Email: yanhan617617@126.com