Journal of Clinical & Experimental Pharmacology

Study on the determination and chiral conversion of R-salbutamol in human plasma and urine by chiral liquid chromatography-tandem mass spectrometry

3^rd World Congress on Pharmacology

August 08-10, 2016 Birmingham, UK

Wen Tan, Ting Zhou, Shan Liu, Ting Zhao, Wenshi Lai and Ling Xu

South China University of Technology, China

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

A sensitive chiral liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of salbutamol enantiomers in human plasma and urine. The chiral conversion of R-salbutamol during the analysis procedure was fully investigated by studying the effects of analysis parameters on the chiral conversion, including pH of the solution, operation temperature, and operation time. Furthermore, the predicted chiral conversion ratio of R-salbutamol calculated by the fitted model under practical conditions was 0.0%, which means the chiral conversion of R-salbutamol determined in this study was not caused by the analysis procedure, but resulted from the in-vivo chiral conversion of R-salbutamol. All the samples were separated on an Astec Chirobiotic T column and detected by a tandem mass spectrometer in multiple reaction monitoring mode. Lower limit of quantification of 0.100 ng/mL was achieved under the optimized conditions. This validated method afforded good linearity, repeatability, accuracy and stability. In addition, this method has been successfully applied to the clinical pharmacokinetic study of R-salbutamol in healthy volunteers. According to the results, this chiral LC-MS/MS assay provides a suitable and robust method for the bioanalysis of salbutamol enantiomers, and the study of the effects of analysis conditions on the chiral conversion has a certain guiding significance for the determination of chiral drugs.

Biography :

Email: went@scut.edu.cn