Journal of Food Processing & Technology

Simultaneous analysis of anti-obesity drugs in food and food supplements using LC/PDA and LC/MS

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Jeung-Young Chai, Mi-Hyune Oh, Moo-Song Lim, Eun-Jung Kim, Joong-Hoon Cho, Chul-Joo Lim and Sun-Ok Choi

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Anti-obesity drugs such as ephedrine, liothyronine (T3), phenolphthalein, levothyroxine (T4) and fluoxetine are illegal to be
included in food since abuse intake of the drugs via food without medical prescription can caused life-threatening side effects.
Governmental agencies of food and drug safety invest their efforts to screen out food and food supplements illegally containing antiobesity
drugs. We developed a simple, rapid and simultaneous analysis method of ephedrine, liothyronine (T3), phenolphthalein,
levothyroxine (T4) and fluoxetine in food and food supplements using liquid chromatography coupled with photodiode array
detection (LC/PDA). Simultaneous analysis of the anti-obesity drugs using a developed method showed a good with respect to
specificity, linearity (r2>0.99), limit of detection, limit of quantification, precision (RSD<8%), recovery (88-97%) and reproducibility.
It also satisfied all standards suggested by AOAC for the analysis of anti-obesity drugs in food and food supplements. To confirm the
detected anti-obesity drugs in food and food supplements, we also developed a qualitative analysis method by Liquid chromatography
coupled with mass spectrometry (LC/MS). Twenty food supplements were tested with the developed method. Anti-obesity drugs were
not detected in all samples. As LC/PDA and LC/MS methods described in this study were simple, rapid and reliable, the methods
may be suitable for a rapid and sensitive analysis of ephedrine, liothyronine (T3), phenolphthalein, levothyroxine (T4) and fluoxetine
simultaneously in food and food supplements.

Biography :

Jeung-Young Chai is a Scientific Officer in Gyeong in Regional Food and Drug Administration of Ministry of Food and Drug Safety.