Journal of Plant Pathology & Microbiology

Showing some broccoli and cabbage genotypes biodiversity using randomly amplified polymorphic DNAs (RAPD)

International Conference on Plant Physiology & Pathology

June 09-10, 2016 Dallas, USA

M M A Abdalla, Aboul-Nasr M H and Shimaa H Mosallam

Assiut University, Egypt

Posters & Accepted Abstracts: J Plant Pathol Microbiol

Abstract:

Ten RAPD markers were used to detect the genetic variability and relationships among 4 broccoli and 3 cabbage genotypes. The results of RAPD analysis showed that all the 5 primers surveyed detected polymorphism for all broccoli genotypes. A total of 39 DNA bands were amplified by the 5 primers from all genotypes and 21 of these fragments showed polymorphism (53.85%). The rest of these bands (46.15%) were common between the 4 genotypes. On the other hand, all of the 7 primers surveyed, used with cabbage, detected polymorphism among all cabbage genotypes. A total of 69 DNA bands were amplified by the 7 primers from all genotypes and 23 of these fragments showed polymorphism (33.33%). The rest of these bands (66.67%) were common between the 3 genotypes. The investigation suggested that the RAPD approach showed considerable potential for identifying and discriminating broccoli and cabbage genotypes.

Biography :

Email: mmalyabdalla@yahoo.com