Journal of Clinical & Experimental Pharmacology

Protective effect of pentoxifilline and their potentiation with nitric oxide modulator in complete Freund's adjuvant induced arthritic changes in rats

World Congress on Pharmacology

July 20-22, 2015 Brisbane, Australia

Rishi Pal, Prafulla Chandra Tiwari, Rajendra Nath and Kamlesh Kumar Pant

Posters-Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Interaction of pentoxifylline with nitric oxide modulators was studied in complete Freund's adjuvant induced rheumatoid arthritis in rats. Wistar rats (200-300 gm, n=6/group) of either sex were used in the study. On day '0' rats were injected with 0.2 ml of complete Freund?s adjuvant (CFA) in sub-planter region of right hind paw along with 0.1 ml of squalene to develop RA while controls received only vehicle. Pentoxifylline treatment alone and in combination with nitric oxide modulators was given from day '14' to '28'. Arthritic parameters (1) arthritis index, (2) ankle diameter, (3) paw volume and (4) their body weight were noted to evaluate progression of RA on day 0, 7, 14, 21 and 28. On day '28' rats were sacrificed and their blood was collected for TNF-? and IL-10 cytokine estimation. Data obtained was analysed using one way ANOVA followed by Newman- Keul?s posthoc test and p<0.05 was considered for significance. It was found that CFA significantly increased arthritis-index, paw volume, ankle diameter and serum TNF-? levels while body weight and serum IL-10 levels was significantly decreased (P<0.05). These CFA-induced changes were significantly reversed by pentoxifylline 5 & 10 mg/kg alone and in combination with L-arginine (100 mg/kg) and L-NAME (10 mg/kg) in dose dependent manner (p<0.005) in all parameters). The maximum protective effects was observed in pentoxifylline 10 mg/kg + L-NAME 10 mg/kg combination treatment group (p<0.001). Results of this study are suggestive of the involvement & interaction of nitric oxide with pentoxifylline which may have a protective role in adjuvant induced RA.