Journal of Clinical & Experimental Pharmacology

Postnatal development and reproductive performance of f1 progeny exposed in utero to an aqueous formulation of Tinospora cordifolia

World Congress on Pharmacology

July 20-22, 2015 Brisbane, Australia

Parag Jain, Sheikh Rayees, Shrawan paswan and Gurudarshan Singh

Posters-Accepted Abstracts: Clin Exp Pharmacol

Abstract:

The present study investigated the development effect of aqueous extract of Tinospora cordifolia commonly known as Guduchi and Giloy. It is an herbaceous vine of the family Menispermaceae. The reproductive toxicity of Tinospora cordifolia was evaluated in rats administered oral doses of 250, 500 or 1000 mg/kg. Twenty rats per sex per dose group were selected in both Parent and F1 generation. In the fertility and early embryonic development to implantation study, male and female rats were treated 2 weeks of pre-mating, 2 weeks of mating and 6 weeks of post-mating in which 3 weeks of Gestation (GD) and 3 weeks of lactation until necropsy. However, in F1 the pups were treated post natal until necropsy including developmental stages of maturation, pre mating, mating and post mating. In the pre- and postnatal development study, pregnant rats were treated from GD 3 weeks through lactation day 3 weeks. Selected F1 pups from this study were evaluated in sensory and behavioral tests and were subsequently mated. No treatment-related effects were observed fetal development, parturition or lactation in the Parent (P) generation. Similarly, no adverse effects of Tinospora cordifolia treatment were observed on preand postnatal growth, development, reproductive performance and embryo-development in the F1 offspring. Based on the results of this study, the No-Observed-Adverse-Effect-Level (NOAEL) for development and reproductive toxicity in rats was considered to be 1000 mg/kg/day, the highest dose tested.