Journal of Clinical & Experimental Pharmacology

Nano-composite of silk fibroin-chitosan/nano diopside for tissue engineering applications: Fabrication and morphology

World Congress on Pharmacology

July 20-22, 2015 Brisbane, Australia

Abbas Teimouri1, L Ghorbanian2 and Alireza Najafi Chermahini2

Posters-Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Tissue engineering uses a scaffold inhabited with signifying molecules and cells to encourage the regeneration of host tissue. Silk fibroin (SF) has attracted important interest in the generation of new materials, because SF is a biocompatible and biodegradable natural polymer with excellent mechanical properties and high chemical reactivity. Chitosan (CS) is a deacetylated product of chitin that can be extracted from crustaceans. Due to its excellent bioactivity and degradability, diopside has been proposed as a potential material for the bone tissue regeneration. In continuation of our recent study on the construction of composite scaffolds, in the present study, Silk (SF), Chitosan (CS) and Nano Diopside were all combined using the freeze drying technique to fabricate a bio-composite scaffold. The composite scaffold (SF/CS/ Nano Diopside) was characterized by SEM, XRD, TGA, BET and FT-IR studies. The scaffold possessed a porous nature with pore dimensions suitable for cell infiltration and colonization. The presence of Diopside in the SF/CS/ Nano Diopside scaffold increased compressive strength and water uptake capacity and decreased porosity. Cytocompatibility of the SF/CS/ Nano Diopside scaffold was assessed by MTT assay revealed non-toxicity to the Human Gingival Fibroblast (HGF, NCBI: C-131(Thus, we suggest that SF/CS/N Nano Diopside composite scaffold is a potential candidate to be used for tissue engineering.