Gautam Patra, D Kathiresan, H Lalrinkima, S K Borthakur and S Ghosh
College of Veterinary Sciences and Animal Husbandry, India
Posters & Accepted Abstracts: J Bacteriol Parasitol
To investigate and identify the nematode found in the body cavity of Cricket in the Mizoram state of North East of India. DNA sequence and Scanning Electron Microscopy (SEM of 18S rRNA of internal transcribe (ITS2) were used for validation of species and fine structure. Based on the morphological and molecular analysis, the nematode infesting Cricket was confirmed to be Gordius species. The length of the amplified ITS2 sequence of Gordius was found to be approximately 1048 bp. This is the first report of the nucleotide sequence of ITS2 of Gordius from India. Phylogenetic analysis revealed that the Gordius species is a close relative (100%) of Malaysian species. Present study also describes the Light Microscopy (LM) and SEM application for detailed morphological study of the isolated species. This is the first report of the occurrence of Gordius in India to the best of the authorsâ knowledge.
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