Journal of Vaccines & Vaccination

Measles virus vaccine infects tumor cells and induces dendritic cells (DC) maturation and tumor antigen cross-presentation

4^th International Conference on Vaccines & Vaccination

September 24-26, 2014 Valencia Convention Centre, Spain

Marc Gregoire, Guillerme Jean-Baptiste, Boisgerault Nicolas, Roulois David, Tangy Frederic and Fonteneau Jean-Fran?ois

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

D endritic Cells (DC) are antigen presenting cells specialized in inducing immune responses. Measles Virus vaccine (MV) was recently proposed as anti-tumor agent to target and kill specifically tumor cells, without infecting healthy cells. We demonstrated that myeloid dendritic cells (mDC) co-cultured with MV infected tumor cells, actively matured and cross- presented tumor antigen. Recently, we also investigated the effects of MV tumor infected cells on phenotype and functions of plasmacytoid DC. We studied maturation, cytokine production and tumor antigen cross-presentation by mDC and pDC exposed either to the virus alone, MV infected or UV irradiated tumor cells. We found that MV infected cells induce DC maturation with a strong cytokine production, notably IFN-α, whereas UV irradiated tumor cells and the MV alone did not. We also observed that MV infected and UV irradiated cells were similarly phagocytosed by DC, although this up-take was less important than in myelo?d DC. Interestingly, we observed cross-presentation of tumor antigen to a specific CD8 + T cell clone only when DC are co-cultured with MV infected tumor cells. Altogether, our results suggest that the use of MV, as anti-tumor virotherapy, may induce immunogenic tumor cell death allowing DC to cross-present tumor antigen. Data will be presented with effects on mesothelioma, melanoma and lung cancer.