Sercan Mercan and Fatma Arslan
Gazi University, Turkey
Aziz Sancar Research Center, Turkey
Posters & Accepted Abstracts: J Diabetes Metab
Type 1 diabetes mellitus (T1DM) is a cell-specific autoimmune disease triggered by selective destruction of the insulin producing �? cells within the pancreatic islets. The transplantation of the islet cells is a promising approach for the treatment of type 1 diabetes on condition of using immunosuppressive drugs. However, there are numerous side effects associated with immunosuppressant drugs. So, transplantation of these islet cells after masking the antigens on the cells surface can solve this problem. Poly amino acids are used in many biomedical areas. In this study, poly-L-glutamic acid modified with biocytin (PLGA-Biocytin) was used for surface modification of islet cells. PLGA-Biocytin structure was elucidated by FT-IR and NMR analysis. PLGA-Biocytin interactions were provided on individual islets via layer-by-layer assembly technique. The surface of the islets is modified with Sulfo-Nhydroxysuccinimide (Sulfo-NHS)-LC-Biotin and the islets are further covered by streptavidin (SA) and PLGA-Biocytin using the layer-by-layer method. In-vitro viability of islet cells and insulin secretion were assessed in both control and PLGA-Biocytin group as a response of exposure to cytokine combination. Viability results of surface modification group islet cells with 48 hours cytokine exposure (52.3%) were found similar with islet cells of control group (55.9%). This method can be considered as developable method for islet cell transplantation.
Email: sercanmrcn@gmail.com
