Journal of Clinical & Experimental Pharmacology

Integrative studies with multidisciplinary approach from standardized extracts in the treatment of peptic ulcer disease

World Congress on Pharmacology

July 20-22, 2015 Brisbane, Australia

Clelia Akiko Hiruma-Lima

Posters-Accepted Abstracts: Clin Exp Pharmacol

Abstract:

The increase in human life expectancy has required the development of new drugs to treat several chronic diseases such as peptic ulcer disease (PUD). The PUD is a complex and multifactorial process including bacterial infections, the increase of acid secretion, generation of reactive oxygen species (ROS), inhibition of the endogenous PGs, and the degradation of the extracellular matrix. This disease has been increasing in elderly people because of the intrinsic weak mucosal barrier induced by aging and also the frequent use of non-steroidal anti-inflammatory drugs (NSAIDs). The last decade has offered new insights in the preventative therapy and also the healing of PUD. The synergistic efficiency of standardized herbal drugs could be the new perspective for treatment of this disease. In our project entitled "Standardized extracts for the treatment of chronic disease", our research group realized integrative studies with multidisciplinary approach with phytochemical, pharmacological and toxicological assays. Based on these integrative studies we evaluated more than 20 medicinal species according to the pharmacopeial standard and evaluated the ensured efficacy and safety of these herbal medicines as antiulcer drug. The antiulcer studies of these extracts were investigated against different ulcerogenic agents including NSAID, HCl, pyloric ligature, absolute ethanol and ischemia?reperfusion procedure. We evaluated the gastroprotective effect of extracts by analyzing the gastric juice secretion, mucus, nitric oxide (NO), sulfhydryl compound, vanilloid receptor, glutathione (GSH) levels, and myeloperoxidase (MPO) activity in the gastric and duodenal mucosa. We also evaluated the gastric and duodenal healing effects of extracts and evaluated the effect of matrix metalloproteinase activity (MMP-2 and MMP-9) and roles of VEGF, PCNA, and COX-2 in cell proliferation.