Inhibitory effect of Corydalis heterocarpa on MMP-2, -9 activity in HT-1080 human fibrosarcoma cell
15th International Conference on Clinical Nutrition
May 24-26, 2018 | Vienna, Austria

Jung Hwan Oh, Ga Hyun Yu and Chang-Suk Kong

Silla University, South Korea

Posters & Accepted Abstracts: J Nutr Disorders Ther

Abstract:

The matrix metalloproteinases (MMPs) have been implicated in normal matrix remodelling events such as mammary gland involution and in pathological conditions such as metastasis of cancer progression. Among them, MMP-2 and MMP9 belong to a group named gelatinase. MMP-2 is associated with tumour tissues, and the appearance of active MMP-2 is closely correlated with tumour metastasis. The halophyte, Corydalis heterocarpa has recently been reported to have important biological implications such as anti-oxidative, anti-inflammatory, and anti-bacterial activities. In this study, the MMP inhibitory effect of C. heterocarpa was evaluated in phorbol 12-myristate 13-acetate (PMA) stimulated HT-1080 cells using crude extracts and their solvent fractions. The organic layer was partitioned between 85% aq. MeOH and n-hexane, and then the aqueous layer was fractionated with n-BuOH and H2O. Effects of ethanol extracts and their solvent fractions on MMP-2, -9 expression were evaluated using gelatin zymography and reverse transcription polymerase chain reaction (RT-PCR). The ethanol extracts and their solvent fractions downregulated PMA-induced MMP-2, -9 expression in a dose-dependent manner. Furthermore, the protein expression levels of MMP-2, -9 were analyzed by western blotting. Presence of crude extract and solvent fractions decreased PMA-induced the MMP-2, -9 activity. Based on current study results, C. heterocarpa was suggested to possess MMP inhibiting natural components indicating that C. heterocarpa may be used as a valuable source of anti-metastasis substances.

Biography :

Jung Hwan Oh has completed her PhD from Tokyo University of Marine Science and Technology and Post-doctorate from North Carolina State University. She has published more than 100 papers in reputed journals.
Email:wjdghks0171@naver.com