Jung Hwan Oh, Ga Hyun Yu and Chang-Suk Kong
Silla University, South Korea
Posters & Accepted Abstracts: J Nutr Disorders Ther
The matrix metalloproteinases (MMPs) have been implicated in normal matrix remodelling events such as mammary gland involution and in pathological conditions such as metastasis of cancer progression. Among them, MMP-2 and MMP9 belong to a group named gelatinase. MMP-2 is associated with tumour tissues, and the appearance of active MMP-2 is closely correlated with tumour metastasis. The halophyte, Corydalis heterocarpa has recently been reported to have important biological implications such as anti-oxidative, anti-inflammatory, and anti-bacterial activities. In this study, the MMP inhibitory effect of C. heterocarpa was evaluated in phorbol 12-myristate 13-acetate (PMA) stimulated HT-1080 cells using crude extracts and their solvent fractions. The organic layer was partitioned between 85% aq. MeOH and n-hexane, and then the aqueous layer was fractionated with n-BuOH and H2O. Effects of ethanol extracts and their solvent fractions on MMP-2, -9 expression were evaluated using gelatin zymography and reverse transcription polymerase chain reaction (RT-PCR). The ethanol extracts and their solvent fractions downregulated PMA-induced MMP-2, -9 expression in a dose-dependent manner. Furthermore, the protein expression levels of MMP-2, -9 were analyzed by western blotting. Presence of crude extract and solvent fractions decreased PMA-induced the MMP-2, -9 activity. Based on current study results, C. heterocarpa was suggested to possess MMP inhibiting natural components indicating that C. heterocarpa may be used as a valuable source of anti-metastasis substances.
Jung Hwan Oh has completed her PhD from Tokyo University of Marine Science and Technology and Post-doctorate from North Carolina State University. She has published more than 100 papers in reputed journals.
Email:wjdghks0171@naver.com