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Immunoneutralization of gonadotrophin releasing hormone (GnRH-I) by using a plasmid DNA vaccine coding GnRH-I can be a potential candidate to supress fertility in mammals
5th Asia Pacific Global Summit and Expo on Vaccines & Vaccination
July 27-29, 2015 Brisbane, Australia

Umme K. Rimaa, Tadashi Kimurab, Valerie A. Ferroc, Mohammad T. Islamd, Abu S.M. Barie and Mohammad A. H. Khane

Posters-Accepted Abstracts: J Vaccines Vaccin

Abstract:

Immunoneutralization of GnRH-I is beneficial to suppress fertility in vivo. To achieve this, we have engineered a plasmid DNA vaccine coding eight repeats of GnRH-I peptide. Here the key focus was to stimulate Th-2 response and enhance immunity by vector incorporation. Sexually mature Swiss albino male and female mice (four in each group) were immunized with 50?g plasmid DNA vaccine in study week 0, 3, 6, 9 and 12. Group 2 female and Group 6 male were primed in Hemagglutinating Virus of Japanese Envelope (HVJE) and boosted in PBS. Group 3 female and Group 7 male were immunized with Non-ionized surfactant vesicle (NISV) through subcutenious route. Group 4 female and Group 8 male were immunized with Bilosome orally. Group 1 females and Group 5 males served as untreated control. In study week 24 significantly higher (p>0.001) anti-GnRH-I antibody (OD value at A540�SD) response ws detected in NISV (0.982�0.231) and HVJE (0.783�0.191) mediated immunization than Bilosome (0.537�0.183) mediated immunization. Immunization of female mice prolonged estrous period and reduced cellular densities in vaginal lavages. Significant reduction of ovarian folliculogenesis was seen in Group 2 (p>0.01) and Group 3 (p>0.001) mice. Vaccinated male mice in Group 6 and Group 7 appeared infertile. Reduction of serum testosterone concentration (ng/ml) in Group 6 (1.575�1.273) and Group 7 (0.625�0.417) male was seen compared to a low affect in Group 8 (4.465�0.959) and unaffected in Group 5 controls (7.268�3.374). The plasmid DNA delivered with HVJE and NISV arrest fertility in males and suppress fertility in females mice.

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