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Immunological assay and arbitration inspection from single to multiple mycotoxins in agro-food
7th Euro Global Summit on Clinical Microbiology and Mycotoxins
February 27-28, 2017 Amsterdam, Netherlands
Zhaowei Zhang and Peiwu L I
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, China
Ministry of Agriculture, China
Posters & Accepted Abstracts: Clin Microbiol
Abstract:
Multiplexed mycotoxins with strong carcinogenesis and toxicity are fatal threats in food and feed safety, and require highly sensitive and high-throughput detections greatly. Rapid immunoassay and arbitration detection methods play a critical role on two sides of one coin. In the rapid immunoassay, a series of high specific and high affinity monoclonal antibody, recombinant antibody and nano-body against aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEA), etc., were developed as the key recognition reagents. Based on these specific antibodies, simultaneous detection for multiplexed mycotoxins was studied by using the Au (or Europium)-based lateral flow strip and non-fouling antigen microarray. The limit of detection was lowered down to pg mL-1 level (0.3 pg mL-1), depending on mycotoxins in food and feed samples. On the other hand, simultaneous arbitration detection method based on LC-MS/MS was investigated. Either multiplexed immuno affinity column or solid phase extraction column was used in the sample extraction. The internal standard allowed precise determination of mycotoxins regardless of matrices. Multiplexed mycotoxins (AFB1, B2, G1, G2, OTA, ZEA and T-2 toxin) were successfully identified by using a new multi-immuno affinity column in a single run. Furthermore, a promising proposal was suggested to achieve the rapid, sensitive, ultra high-throughput detection of 96-384 contaminants in food and feed, including bio toxins, pesticides, veterinary drugs, etc., based on immuno chemiluminescence biosensors using Hadamard transformation imaging (iHT).
Biography :
Email: zwzhang@whu.edu.cn