Saeed Nazeri, Sedigheh Zakeri, Akram A Mehrizi and Navid D Djadid
Pasteur Institute of Iran, Iran
Posters & Accepted Abstracts: J Bacteriol Parasitol
Statement of the Problem: The thrombospondin-related adhesive protein (TRAP) is one of the major sporozoite antigens that play an important role in the invasion of mosquito salivary glands and hepatocytes by sporozoites. A key tool for the control, elimination and eradication of Plasmodium vivax is the development of an effective vaccine. Therefore, it is very essential to assess the natural immune responses to a particular antigen in diverse populations with different genetic background and from various malaria-endemic settings. Methodology & Theoretical Orientation: For our purpose, naturally acquired immune responses to PvTRAP was evaluated in patients from malaria-endemic areas of Iran (n=116). The PvTRAP gene was expressed in Escherichia coli and used as antigen in enzyme-linked immunosorbent (ELISA). The profile of immunoglobulin G (IgG) isotype and the avidity of IgG, IgG1 and IgG3 to PvTRAP, as well as the association between anti-PvTRAP isotype responses and host age were evaluated. Findings: The results showed that only 42.24% of Iranian patients infected with P. vivax had positive anti-PvTRAP IgG. Furthermore, the positive responses of IgG1 and IgG3 antibody responses to PvTRAP revealed no significant correlation with age (p>0.05). Conclusion & Significance: Individuals exposed to vivax malaria in the unstable malaria transmission areas are capable to produce antibodies to the PvTRAP antigen at all ages. The present data also suggests the presence of mature IgG1 and IgG3 antibodies with high to intermediate avidity against PvTRAP antigen that could help to understand the interactions between the host and P. vivax parasite in developing and testing a TRAP-based vaccine.
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