Journal of Food Processing & Technology

Hydrolytic enzymes production by solid state fermentation of nopal with Trichoderma harzianum and Trametes polyzona

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Jimenez Sanchez M2, Mondragon Rojas A G1, Perez Diaz D1, Rincon Reyna J1, Rincon Reyna P1, Ortiz Moreno A1, Jimenez Garcia E1 and Sanchez Pardo M E1

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Agricultural crop residues of nopal are rich in cellulose, hemicellulose and lignin given its composition can be used as
substrates in a process of solid state fermentation (SSF) with filamentous fungi (Ascomycetes and Basidiomycetes) in
order to produce extracellular enzymes with hydrolytic activities of commercial interest. The main aim of this research was to
describe the production of cellulase and xylanase enzymes by Trichoderma harzianum (Ascomycete) and Trametes polyzona
(Basidiomycete) by solid state fermentation using vegetable nopal paddle and prickly pear paddle as substrates. An indirect
measure of enzyme production was determined the total protein content (Bradford method) and the enzyme activities by the
DNS method every 24 hours during 8 days by Trichoderma harzianum and 10 days by Trametes polyzona. For Trichoderma
harzianum the activity of xylanase enzyme increased on vegetable nopal paddle with 2.34 U/gdm (gram of dry matter) on the
5th day of fermentation while the prickly pear paddle turned out to be a better substrate for the cellulase activity with 0.57 U/
gdm the 3rd day. For Trametes polyzona, the activity of both enzymes increased and the highest activity was yield on the 5th day of
fermentation on vegetable nopal paddle obtaining values of 4.38 U/gdm for xylanase and 1.00 U/gdm for cellulase. The highest
activity of both enzymes was observed with Trametes polyzona using vegetable nopal paddle as substrate then it is possible to
use this fungi in order to hydrolyze residues of nopal by solid state fermentation.

Biography :

Jimenez Sanchez M is currently from Tecnológico de Monterrey Estado de Mexico.