Genotype I and IV of dengue virus DEN-1 and its clinical manifestasions in Surabaya
4th International Conference on Clinical Microbiology and Microbial Genomics
October 05-07, 2015 Philadelphia, USA

Puspa Wardhani, Benediktus Yohan, Aryati, Tri Yuli Setianingsih, Dwiyanti Puspitasari, Muhammad Vitanata, Soeharto and R Tedjo Sasmono

Universitas Airlangga, Indonesia
Eijkman Institute for Molecular Biology, Indonesia

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

Previous studies showed there were a Dengue Virus (DENV) distribution shift from DENV-2 to DENV-1 in Surabaya. This study analyzed genetic characteristics of DENV-1 and clinical manifestations. This was an analytical observational study with prospective cohort setting. The study was conducted in Dr. Soetomo General Hospital, from February to August 2012. DENV serotyping was done by real-time PCR using SimplexaTM 3M RT-PCR instrument. Viral load examination was done by two step real-time PCR in Applied Biosystem 7500 instrument. Positive samples were cultured in C6/36 cells. Positive culture samples were genotyped using envelope gene sequence. Dengue serotype distributions in 2012 were DENV-1 (67.9%), DENV-2 (9.5%), DENV-3 (4.8%), DENV-4 (7.1%), mixed DENV-1 & 2 (2.4%), DENV-1 & 3 (5.9%), and DENV-1 & 4 (2.4%). DENV-1 consisted of genotype I (66.7%) and IV (33.3%), genotype II, III and V were not detected. Comparation among DENV serotypes or DENV-1 genotype showed no significant differences in DF and DHF manifestations. In the children group, red blood cell count (RBC) was higher in DENV-1 genotype I than IV, but mean corpuscular hemoglobin (MCH), lymphocytes and albumin level were lower in genotype I. Viral load level was higher in genotype I than IV, unfortunately it was not significant. By analyzing E gene nucleotids sequences, each DENV-1 strain showed individual nucleotides and amino acid changes. From E gene sequences analysis, amount of amino acid subtitutions had no implication in DF and DHF manifestations.

Biography :

Puspa Wardhani is a Medical Doctor, finished Clinical Pathologist brevet from Airlangga University and PhD degree in 2013. She is Lecturer in Airlangga University and Medical Staff in Dr. Soetomo General Hospital in Surabaya. She is currently undergoing subspecialized brevet of microbiology and infectious disease. She has international journal publication and has been serving as an Editor Chief of local journal in Indonesia.

Email: puspa_pk@yahoo.co.id