Agrotechnology

Genetic fingerprinting of root knot nematode (Meloidgyne spp) as serious pest in crop production in Egypt

7^th Global Summit on Agriculture & Horticulture

October 17-19, 2016 Kuala Lumpur, Malaysia

Sanaa A Haroon and Mary Zaher

Fayoum University, Egypt

Posters & Accepted Abstracts: Agrotechnol

Abstract:

Nematodes Meloidogyne spp are major pests all over the world and causes diseases to economically important crops. The annual loss worldwide is approximately 82 billion dollar. Samples were collected from different crops all over Egypt. Nematode samples identified by using two polymerase chain reaction which are (rDNA) and (mtDNA). The RAPD technique was used to investigate the genetic diversity of these nematodes. The common populations were M. incognita and M. javanica and were distinguished by differences in fragment patterns with OPB3, OPB11, OPB17 and OPG6. Two RAPD markers were detected; one was specific for M. incognita population with primer OPK2 at fragment size of 1000bp and the second was specific for M. javanica population with primer OPB3 at size of 1100bp this two markers were used as SCAR markers, which were sequenced and two PCR primer pairs designed for M. incognita and M. javanica. The amplification of mtDNA is used to distinguish genera and species of root knot nematode. Primer annealing sites were located in the 3 portions of the mitochondrial gene coding for cytochrome oxidase subunit II the 16 S rRNA gene following PCR amplification fragment of size 1700bp specific for genus meloidgyne was produced. Digestion of the amplified product with restriction enzymes allowed discrimination among different meloidgyne species with identically sized amplification products. Hinf I digestion of the 1700bp fragment produce two bonded patterns in M. javanica and three bonded patterns in M. incognita, that help in investigating genetic diversity also identifying molecular markers characteristics of nematodes (M. javanica and M. incognita)

Biography :

Email: sanaaharoon1951@gmail.com