Talita Antunes Guimar?es
Posters: J Diabetes Metab
Introduction: Gallic acid (GA) is a trihydroxybenzoic acid present in plants worldwide. Gallic acid has been shown to have cytotoxic effects in certain cancer cells, without damaging normal cells. The objective of the present study was to determine whether gallic acid is able to inhibit oral squamous cell carcinomacell viability, proliferation and invasion and suppresscell‑mediated angiogenesis. Treatment of oral squamous cell carcinoma (SCC9 and SCC4) with gallic acid decreased cell viability in a dose‑dependent manner. Methods and Results: We first confirmed that GA decreases proliferation, invasion and migration in squamous cell carcinoma lines. Next, SCC-9 and SCC-4 cells were incubated in a well humidified incubator with 5% CO2 and 95% air at 37 ?C (normoxiccondictions). For hypoxic cultures, cells were incubated 150 μM CoCl2 for 24 hours and maintained in a 5% CO2 atmosphere at 37˚C. Then, we divided cells into 4 groups including control, CoCl2, GA (10μg/mL) and CoCl2 + GA (10μg/mL). Col1A1 mRNAwas decreased in hypoxic cells after treatment with GA. Besides, GAincreased HIF-1α and e-cadherin mRNAs levels. In conclusion, our current findings suggest that GAincreased HIF-1α and e-cadherin mRNAs levels, and decreased Col1A1 mRNA levels. In addition, we found that the GAinhibit oral cancer cell migration, proliferation and invasion.
