Journal of Neonatal Biology

Expression of programmed death-1 receptors in the monocytes isolated from the newborns with birth weight â?¤1500 g

9^th International Conference on Neonatology and Pediatric Neurology

November 28-30, 2016 Valencia, Spain

Magdalena Zasada, Nina Mol, Marzena Lenart, Magdalena Rutkowska Zapala, Maciej Siedlar and Przemko Kwinta

Jagiellonian University, Poland

Posters & Accepted Abstracts: J Neonatal Biol

Abstract:

Aim: The aim of the study was to compare the expression of programmed death-1 (PD-1) receptors on the monocytes isolated from the newborns with birth weight�?�1500 g during either non-septic time or late-onset sepsis (LOS). Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 55 patients with birth weight�?�1500 g without earlyonset sepsis on the 5th day of life (DOL). PD-1 expression was measured on the monocyte subsets by flow cytometry. If a patient developed LOS, same analysis was repeated. Further studies were performed after subdividing children into born�?�27 and �?�28 gestational week (GW). Results: No differences in the percentages of monocytes that expressed PD-1 were found on the 5th DOL between newborns born �?�27 and �?�28 GW. However, in the group born �?�27 GW, the patients who did not develop LOS, had higher percentage of intermediate monocytes with PD-1 receptor expression in comparison to newborns, who later developed LOS (19.5% vs. 7.26%; p=0.0142). Moreover, we observed further reduction of intermediate monocytes with PD-1 expression comparing to the initial values during LOS (3.54% vs. 7.26%; p=0.0161). In contrast, there was an increase of the total count of intermediate monocytes with PD-1 expression during LOS in the group of infants born after 28 GW (9IQR6-16 cells/ul vs. 25IQR15-38 cells/ul: p=0.0263). Conclusion: We demonstrated that PD-1 receptors were present on the monocytes isolated from the preterm newborns with birth weight�?�1500 g. The infants born �?�28 GW showed more intermediate monocytes with expression of PD-1 receptors during LOS. Further studies are needed to establish the role of PD-1 during sepsis in babies born very preterm.

Biography :

Email: magdamedic@gmail.com