Journal of Aquaculture Research & Development

Evaluation of immunogical genes at gill of Ictalurus punctatus immunized with live theronts of Ichthyophthirius multifiliis

3^rd International Conference on Aquaculture & Fisheries

September 29-October 01, 2016 London, UK

Gabriel S. A. Moreira, De-Hai Xu, Craig A. Shoemaker, and Dunhua Zhang

Laboratory of Parasitology, College of Animal Science and Food Engineering, University of São Paulo (USP), Brazil.
United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, USA

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

In aquaculture systems, fish are commonly infected by parasite Ichthyophthirius multifiliis (Ich) that can result in heavy economic losses for aquaculture. There is limited information on innate and adaptive immune gene expression in the gill of channel catfish, Ictalurus punctatus immunized with Ich theronts. The objective of this study is to evaluate differential expression of innate and adaptive immune genes, including immune cell receptor, immunoglobulin, recombination-activating gene (RAG), cytokine and inflammatory protein in gill from channel catfish at different times after immunization with live theronts of Ich. In the adaptive response, the T cell receptor TCR-α and TCR-β showed a similar pattern of expression with a peak of up-regulation at D20. The immunoglobulin IgM exhibited an up-regulation at all time point while the IgD showed a down-regulation at 4h. The peak of upregulation of both Igs was observed at D10. Expression of RAG1 and RAG2 exhibited a rapid increase with a peak of up-regalation at D10 and 4h respectively. The gene expression of innate system such as cytokine (IL1-βa, IL1-βb, IFN-γ and TNF-α) exhibited a peak of up-regulation at D1 post imunization. Inflammatory protein lysozyme-G showed high up-regulation at D20 but both genes COX- 2 and transferin did not showed an significant up-regulation. This study demonstrated genes expression at the gill of channel catfish involved in innate and adaptive response against Ich following live theront vaccination.
This research was supported by USDA/ARS CRIS Project #6010-32000-026-00D and FAPESP, Brazil

Biography :

Gabriel S. A. Moreira is a graduatein Biological Sciences from the Centro Universitário de Ensino Octavio Bastos, Brazil by arrangements in 2008 and a BA Degree in 2009. In 2013 completed the Master USP in the campus of Animal Science and Food Engineering program at Animal Science, Brazil. Currently he is a PhD student at the same universty where he is developing the study on the expression of genes in Pseudoplatystoma corruscans, Pseudoplatystoma reticulatum, and its hybrid immunized with Ichthyophthirius multifiliis. Has experience in parasitology, molecular biology and immunology