Evaluation of high performance liquid chromatography, gas chromatography mass spectrometry of fractions of Viburnum opulus (L) and toxicological studies on male albino Wistar rats
3rd World Congress on Pharmacology
August 08-10, 2016 Birmingham, UK

A H Adebayo and A B Alade

Covenant University, Nigeria

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

This study was conducted to evaluate the phytochemical constituent of Vibrunum opulus (L) with the aid of qualitative screening, high performance liquid chromatography (HPLC) and gas chromatography mass spectrometry (GC-MS). The study also evaluated the effect of V. opulus on different strains of micro-organism and the safety potential of the leaf on albino Wistar rats (AWR) using biochemical and histological indices of toxicity. Ethanol, n-hexane, ethyl acetate, butanol and water fractions were prepared for both phytochemical and anti-microbial activity. Five groups of seven male AWR per group were used for the study. Group A was administered with distilled water and was used as the control group, while groups B, C, D, E were respectively administered with 250, 500, 1000 and 1500 mg/kg body weight of the ethanolic leaf extract of V. opulus by gastric intubation for 28 days. Phytochemical screening revealed the presence of carbohydrate, tannins, saponin, alkaloids, terpenoids, coumarins, phenols, caryophyllene, germacrene D, naphthalene, diethyl phthalate, neophytadiene, butane,1,1-dibutoxy-, pentadecane, 2,6,10,14-tetramethl, octadecane, and tridecane screened with the aid of HPLC as obtained from GCMS. Animals were subsequently anesthetized, blood samples were collected for biochemical assays; organs were isolated and weighed, while the liver tissues were processed for histopathological studies. Alkaline phosphatase (ALP), high density lipoprotein, low density lipoprotein, cholesterol and total protein were significantly (p<0.05) elevated in animals administered with 500, 1000 and 1500 mg/kg body weight of the extract. Ethanol, butanol and aqueous fraction of the leaf of V. opulus showed significant effect on Klebsiella pneumoniae pneumonia and Klebsiella ozaenae strain of microorganism. Histopathological studies conducted revealed that there was no significant damage on the liver tissues. The result suggests that extract of the leaf of V. opulus contains a wild range of fatty acids, heterocyclic compound which have antimicrobial properties, so that it can be recommended as a plant of phytopharmaceutical importance and the extract of the leaf of V. opulus could alter some biochemical parameters and may not cause any adverse effect on the liver tissue.

Biography :

Email: abiodunadebayo@cu.edu.ng aristotlealade@gmail.com