Journal of Clinical & Experimental Pharmacology

Effects of selective PDE-2 inhibitor BAY60-7550, PDE-5 inhibitor Sildenafil and PDE-9 inhibitor PF- 04447943 on learning and memory in the passive avoidance test in naive mice

8^th World Congress on Pharmacology and Toxicology

July 24-25, 2017 Melbourne, Australia

Guner Ulak, Emine Bektas, Furuzan Yildiz Akar, Oguz Mutlu and Faruk Erden

Kocaeli University, Turkey

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Phosphodiesterases (PDE) are enzymes that hydrolyze cAMP and/or cGMP throughout the body, including the brain. Preventing the breakdown of cAMP by PDE inhibitors promotes memory. PDE inhibitors present a novel therapeutic approach with which to arrest cognitive decline or possibly reverse the decline with cognition enhancement. The aim of this study was to investigate effects of BAY 60-7550 (a PDE2 inhibitor), Sildenafil (a PDE5 inhibitor) and PF-04447943 (a PDE9 inhibitor) on emotional memory in naive mice using Passive Avoidance (PA) test. Mice were trained in a one trial stepthrough PA apparatus for evaluating emotional memory; whereas, decrease in retention latency indicate an impairment of memory. BAY60-7550 (1 and 3 mg/kg), Sildenafil (3 and 10 mg/kg) or PF-04447943 (1 and 3 mg/kg), (n=12-15 per group) was administered intra-peritoneally 30, 60 and 60 minutes, respectively before the acquisition session of PA test. One way Anova post hoc Tukey��?s test was used for the statistical analysis of the data. The results of this study revealed that there was significant difference between the first day latency of the animals. Drug treatment, significantly prolonged the retention latency of PA test in the second day compared to control group (3 mg/kg BAY 60-7550 vs. control p<0.05, 10 mg/kg Sildenafil vs. control p<0.05, 3 mg/kg PF-04447943 vs. control p<0.01). Our results confirm that BAY 60-7550, Sildenafil and PF-04447943, enhance emotional memory of mice in the PA test. Further studies and different cognition models are needed to support our results and enlighten whether these effects are test dependent or not.

Biography :

Guner Ulak has completed her PhD from Dicle University, School of Medicine and Postdoctoral studies from Kocaeli University, School of Medicine, Turkey. She is the Director of Pharmacology Department, Kocaeli University, Turkey. She has published more than 70 papers in English, 45 in Turkish and has given many poster presentations.

Email: gunerulak@yahoo.com