Journal of Clinical & Experimental Pharmacology

Effects of anandamide and agmatine on cisplatin-induced neuropathy

8^th World Congress on Pharmacology and Toxicology

July 24-25, 2017 Melbourne, Australia

Kevser Erol, Bilgin Kaygisiz, Cigdem Cengelli, Sule Aydin, Basak Donertas, Engin Yildirim and Emel Ulupinar

Eskisehir Osmangazi University, Turkey

Scientific Tracks Abstracts: Clin Exp Pharmacol

Abstract:

Objective: Cisplatin is a commonly used antineoplastic agent for the treatment of several solid tumors. Peripheral neuropathy is one of its major side effects. Agmatine has been shown to relieve neuropathic pain in different animal models. Cannabinoids have also shown analgesic features in neuropathic pain models. The aim of this study was to investigate the effects of anandamide, a cannabinoid receptor agonist and agmatine on cisplatin-induced neuropathy. Materials & Methods: Neuropathy was induced in male Wistar rats (250-300 gm, n=6) by intra-peritoneal injections of cisplatin (3 mg/kg once a week for five consecutive weeks). The development of neuropathy was evaluated using tests for both mechanical allodynia and heat-hypo/hyperalgesia. After 5 weeks of treatments, cardiac perfusion with para-formaldehyde was performed and Dorsal Root Ganglion (DRG) neurons were collected for further electron microscopic evaluation. Single dose of agmatine (100 mg/kg) or anandamide (1 mg/kg) was given intra-peritoneally. The development of neuropathy was evaluated using tests for both mechanical allodynia and heat-hypo/hyperalgesia. Spontaneous locomotor activity, rectal temperature, anti-nociception and catalepsy were evaluated for cannabinoid tetrad. After 5 weeks of treatments, cardiac perfusion with para-formaldehyde was performed and Dorsal Root Ganglion (DRG) neurons were collected for further electron microscopic evaluation. Results: Cisplatin treatment induced mechanical allodynia and thermal hyperalgesia. Single dose of agmatine significantly attenuated cisplatin-induced mechanical allodynia. Conclusions: It seems that agmatine may have anti-nociceptive activity on cisplatin-induced neuropathy. However, single dose of anandamide did not alleviate cisplatin-induced thermal hyperalgesia.

Biography :

Kevser Erol has completed her PhD from Dicle University and Postdoctoral studies from Anadolu University School of Medicine and is the Director of Department of Pharmacology. She has published more than 125 papers in reputed journals.

Email: kerol@ogu.edu.tr