Journal of Nanomedicine & Nanotechnology

Effect of multi-walled carbon nanotubesin three different dispersants on MC3T3-E1 cell line proliferation

5^th International Conference on Nanotek & Expo

November 16-18, 2015 San Antonio, USA

Hisao Haniu

Shinshu University, Japan

Posters-Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Multi-walled carbon nanotubes (MWCNTs) have been reported as both a useful and hazardous biomaterial. In the present study, we examined the relationship between MWCNTs and MC3T3-E1mouse preosteoblast cell line proliferation. MWCNTs were dispersedinthree separate dispersants: FBS, gelatin, and CMC. Cell viabilitywasthen measured under various culture conditions and cell morphology was analyzed.We observed proliferation inhibition under specific conditions only, such as when MC3T3-E1 not cultured incalcification medium wasexposed to MWCNTs dispersed ingelatin. However, MWCNTs dispersed in FBS or CMC did not adversely affect cell proliferation and was seen to significantly promote it under certain conditions. Furthermore, cell proliferation increased with all dispersants when the cells were cultured in calcification medium. Our results demonstratethat MWCNTs are generally of benefit as a biomaterial for bone substitution.

Biography :

Email: hhaniu@shinshu-u.ac.jp