Development of streptozotocin-induced type 1 diabetes in mice exposed to Ascaris suum adult and egg homogenates
5th International Conference on PARASITOLOGY & MICROBIOLOGY
July 12-13, 2018 Paris, France

Zion Carlo A Martinez and Ma Dalisay G Maligalig

University of the Philippines Los Banos, Philippines

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Introduction: The effect of exposure to Ascaris suum adult and egg homogenates on the development of streptozotocin (STZ) induced-diabetes in mice was conducted. Materials & Methods: 40 individual mice (20 male:20 female) were randomly distributed into five groups: Normal Control, Diabetic Negative Control, Adult-Male Homogenate, Egg Homogenate, and Combined Adult-Egg Homogenate. All treatments were given intraperitoneally (IP) once daily for three days at a volume of 10 ml Kg-1 body weight. Diabetes was induced in all mice except those in the Normal Control group, through the IP administration of STZ at 150 mg Kg-1 body weight. Blood glucose was monitored. At the end of the 10-day observation period, urine was collected and analyzed colorimetrically for the presence of bilirubin, ketone bodies, leukocytes and glucose. Animals were sacrificed and the pancreas removed and subjected to histological assay. Results: Results showed that all mice dosed with STZ developed diabetes on the 6th day post STZ. The urine analysis showed no significant differences among treatment groups for all parameters tested. Histological analysis of the pancreas revealed destruction of the islets of Langerhans in mice from the STZ-treated groups. Conclusion: The study suggests that Ascaris suum adult and egg homogenates did not suppress the development of type 1 diabetes.