Clinical Microbiology: Open Access

Development of PCR-ELISA for specific and sensitive detection of Mycoplasma genitalium

7^th Euro Global Summit on Clinical Microbiology and Mycotoxins

February 27-28, 2017 Amsterdam, Netherlands

Arghavan Mokari Golkhatmi, Hadi Farsiani, Lena Goshayeshi, Hoda Radmanesh and Saeid Amel Jamehdar

Imam Reza Hospital-Mashhad University of Medical Sciences, Iran
Antimicrobial Resistance Research Center-Mashhad University of Medical Sciences, Iran

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Mycoplasma genitalium is a pathogenic bacterium that lives on the urinary and genital tracts in humans and can cause significant morbidity in men and women. Related infections are often asymptomatic. There are potentially serious complications that can result from an untreated infection. Routine screening of high-risk individuals would be critical for the detection and treatment of M. genitalium. In the absence of adequate and reliable culture and approved commercial assay techniques, most laboratories use in-house nucleic acid amplification tests for detection of this bacterium. This study aimed to develop a sensitive molecular method of PCR -ELISA for detection of M. genitalium and alternatively determine the prevalence of infection in women referred to a referral infertility Center in Mashhad, Northeast of Iran. Sampling process was done using vaginal swab from 100 infertile women and 100 fertile women as a control group. Isolation of M. genitalium DNA from clinical samples was performed using DNA extraction kit. Primers and biotinylated probe was designed for amplification of MgPa and 16S rRNA genes. For labeling PCR products, we used digoxigenin labeled nucleotides. Hybridization was done in streptavidin coated plate and detection was carried out by ABTS substrate. In this study, prevalence of M. genitalium in infertile women and pregnant women was 21% and 3.3%, respectively; which was statistically significant (p=0.024). Due to high sensitivity and cost effectiveness of PCR- ELISA method in detection of M. genitalium infection, this method can be used as an efficient screening method for diagnosis of this infection routinely.

Biography :

Email: a_golkhatmi@yahoo.com